Coding

Part:BBa_K2845004:Design

Designed by: Antonio Martínez   Group: iGEM18_ETH_Zurich   (2018-10-10)


C-terminus of split EPIC luciferase under strong promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 495
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 199


Design Notes

C-terminus of split EPIC Luciferase (BBa_K325101) under the control of a strong Anderson promoter and a medium RBS. Splitting was performed according to Luker et al.


Source

Luker, Kathryn E., et al. “Kinetics of Regulated Protein – Protein Interactions Revealed with Firefly Luciferase Complementation Imaging in Cells and Living Animals.†PNAS, vol. 101, no. 33, 2004.